Science

What is the difference between ESI and APCI?

Although a charged analyte can be produced via positive ESI and APCI, the ionization process is substantially different in each case. Protonation of the analyte happens in the liquid phase while using ESI. In APCI, the analyte in the gas phase receives a proton from a protonated solvent molecule.

What is APCI in LCMS?

2 Atmospheric Pressure Chemical Ionization (APCI) APCI uses heat and a nebulization gas to form an aerosol of the eluent from an LC system. In contrast to ESI, ions are not formed in solution or liquid phase.

What is the difference in between ESI and ESI?

Hello, ESI means electrospray ionisation so ESI is ionisation source so ESI-MS/MS means LC/MS/MS with ESI electrospray ionization source. LS-MS/MS means liquid cromatograph with triple quad masspectrometer. So if you se LC/MS/MS it system without mention of ionization source. LC/MS/MS ionisation source can be.

What is APCI in mass spectroscopy?

Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry which utilizes gas-phase ion-molecule reactions at atmospheric pressure (105 Pa), commonly coupled with high-performance liquid chromatography (HPLC).

What is corona discharge in APCI?

Corona Discharge (CD) is a widely studied and applied electrical phenomenon where a gas surrounding a high voltage electrode forms an ionized gaseous plasma [1, 2]. Most mass spectrometrists are familiar with corona discharge in the context of APCI, where it is used to create an ionizing plasma [3].

How does ESI MS work?

ESI uses electrical energy to assist the transfer of ions from solution into the gaseous phase before they are subjected to mass spectrometric analysis. Ionic species in solution can thus be analysed by ESI-MS with increased sensitivity.

Who is eligible for ESI?

As for workers or employees, they are covered or entitled under ESI when they earn less than Rs. 21,000 per month and Rs. 25,000 in the case of a person with disability. The worker contributes 1.75% of their salary while the employer contributes 4.75% towards the ESI scheme.

Which is better ESI or health insurance?

ESIC does provide coverage in the case of an emergency; however, the group health insurance policy provides additional benefits that are not covered under the ESIC. Moreover, the employee also pays a certain amount from their salary for ESIC. But under Group medical plan, the premiums are paid by the employer.

Can I continue ESI after leaving job?

In order to reactivate your ESI Pehchan card, you need to give your old ESIC number to your new employer. Your ESIC card will be reactivated when your new employer pays your ESIC contribution.

What is APPI in MS technique?

Atmospheric pressure photoionization ionization (APPI) is a soft ionization technique for liquid chromatography-mass spectrometry (LC-MS) that uses photochemical action to ionize samples in the gas phase. The APPI facilitates the analytical detection of weakly polar and non-polar compounds by mass spectrometry.

What is isotopic peak in mass spectrometry?

Share. Many elements have the natural isotopes. For example, Chlorine with mass number 37 exists in addition to Chlorine 35. The presence of isotopes readily produces the isotope ions in the spectrum accompanied by a main molecular ion peak and fragment peaks.

What is the salary limit for ESI 2021?

All employees of a covered unit, whose monthly incomes (excluding overtime, bonus, leave encashment) does not exceed Rs. 21,000 per month, are eligible to avail benefits under the Scheme. Employees earning daily average wage up to Rs. 176 are exempted from ESIC contribution.

Can we have 2 ESI card?

when your generating ESI Challan at time you need Paste and import from last company. Dear Mandeep, If any employee have get smart card one time, he can not apply again for smart card with different Insurance number, So its solution is that you can register him in ESIC with his previous insurance numbers.

Can we withdraw ESI amount?

ESI is a premium paid for medical benefits. If no benefit is obtained from it, there is no option available to withdraw amount from ESI Account.

Is ESI and mediclaim same?

ESI and medical insurance are totally different thing. ESI is a statutory obligation of the employer whereas medical insurance is not. If the area where your organization is located is covered under ESI then your organization is bound to give ESI to its employees whose gross salary is less or equal to 15000.

Is family covered under ESI?

Dependants benefit (family pension) is payable to dependants of a deceased insured person where death occurs due to employment injury or an occupational disease. A widow can receive this benefit on a monthly basis for life or till her re-marriage. A son or daughter can receive this benefit till eighteen years of age.

What is ESI and its benefits?

This scheme is designed to provide protection to employees, as defined in the Employees' State Insurance Act, against the events of sickness, maternity, disablement and death on account of employment injury and extend medical care to persons insured under the Act and their families.

How is ESI calculated in salary?

Computation of ESI Currently, the employee contribution is 0.75% of wages paid/payable, and employer contribution is 3.25% of wages paid/payable. Let us say Mr Hard Working with wages of Rs. 18,000 work in a factory unit.

What is ESI in salary?

Employee State Insurance (ESI) scheme is a social security scheme that insures employees of establishments that qualify under the ESI Act. The ESI contribution in respect of an employee comprises both the employer's contribution and the employee's contribution.

Can I claim ESI in private hospital?

Beneficiaries of the ESI health insurance scheme can get medical care at nearby private hospitals empanelled with the Employees' State Insurance Corporation in case of non-availability of ESIC hospitals within 10 kms of their residence, an official statement said.

What is the difference between hard ionization and soft ionization?

Hard ionizers produce ions with a great deal of excess internal energy leading to fragmentation. Hard ionizers less likely to produce the molecular ion, M+. Soft ionizers produce considerably less fragment ions and are very likely to produce the molecular ion or a quasi molecular ion.

Is ESI soft ionization?

ESI source is another important soft ionization method used for biomolecule analysis (Figure 12.3). In this method, liquid samples are used. Modern MS instruments are integrated with liquid chromatography. Peptide fragments after trypsin digestion and are purified using liquid chromatography.

Why is ESI good for studying proteins?

ESI MS is particularly useful for characterizing intact proteins, as it produces multiply charged ions whose m/z values are significantly lower than their masses. As a result, detection of these ions can be accomplished using a variety of mass analyzers with relatively low m/z limits.

What is corona in electrical?

DEFINITION: The ionization of air surrounding the high voltage transmission lines causing the conductors to glow, producing a hissing noise, is called Corona Discharge or Corona Effect.

What is corona effect in ESP?

In an ESP, a positive corona had higher oxidation rate than a negative corona because negative oxygen ions would be attracted by the positive discharge wire (Yehia and Mizuno, 2005). It has been reported that oxidation rate constant of stainless steel increased with increasing temperature (Wild, 1977).

What is corona loss in transmission line?

Corona appears in the transmission line when the surface voltage gradient at the line conductor reaches the breakdown stress. Due to corona, heat and bluish light produce. There is a loss of power and energy dissipation. This loss is known as the corona loss.

Can I add my parents in ESI?

i) You are covered by Section 2 (11) (v) of the The Employees' State Insurance Act, 1948, reproduced above. ii) Section 2 (11) (v) DOESNOT distinguish between a married / unmarried / son / daughter. It applies to everyone in the same manner. iii) Your dependent parents will be covered under the ESIC Scheme.

Is ESI mandatory?

Staying compliant with Employee State Insurance (ESI)ESI is mandatory for employers who have employees working in a non-seasonal factory with more than 10 employees, but only for employees who are earning less than ₹21,000 per paycheck.

What is the current ESI limit?

The existing wage limit for coverage under the Act effective from 01.01. 2017 is Rs. 21,000/- per month (Rs. 25,000/- per month in the case of Persons with Disability).

Is ESI mandatory for salary above 15000?

The logic is that since salary for ESI coverage limit is Rs 15,000 per month, there is no need to calculate ESI on any amount above Rs 15,000.

Is ESIC applicable for 25000 salary?

Yes. All the establishments covered under the ESI Act and all factories that employ more than 10 employees and pay wages below Rs. 21,000 per month (Rs. 25,000 for employees with disability) must register with the ESIC and contribute towards the ESI scheme.

How much PF is cut in salary?

You and your employer need to transfer 10% or 12% of your basic salary to contribute towards EPF. However, if you are a woman, you only need to contribute 8% of your basic salary for the first three years. During this period, your employer's EPF contribution will remain 12%.

Is ESIC and UAN number same?

New Delhi, 10 Dec (KNN) The Ministry of Labour and Employment has announced that the Employees' State Insurance (ESIC) Insurance number will be linked to the insured person's EPFO Universal Account Number (UAN), a 12 digit identification number allotted by Employees' Provident Fund Organisation (EPFO).

What is current rate of PF and ESI?

The latest revision is w.e.f. 01.07. 2019 and the rates are as follows: Employer's Contribution – 3.25% of the wages paid/payable. Employee Contribution – 0.75% of the wages paid/payable.

How do you identify isotopes in mass spectrometry?

The relative abundance of each isotope can be determined using mass spectrometry. A mass spectrometer ionizes atoms and molecules with a high-energy electron beam and then deflects the ions through a magnetic field based on their mass-to-charge ratios ( m / z m/z m/z ).

What does m1 peak mean?

If you had a complete (rather than a simplified) mass spectrum, you will find a small line 1 m/z unit to the right of the main molecular ion peak. This small peak is called the M+1 peak.

What is metastable peak?

mass spectrometrypeak is known as a metastable peak. Generally, metastable peaks occur at nonintegral mass numbers, and, because there usually is a kinetic energy of separation during fragmentation of the polyatomic ion, they tend to be more diffuse than the normal mass peaks and thus are recognized easily.

What does the M 2 peak mean?

This page explains how the M+2 peak in a mass spectrum arises from the presence of chlorine or bromine atoms in an organic compound. It also deals briefly with the origin of the M+4 peak in compounds containing two chlorine atoms.

What is Rule of 13 in mass spectrometry?

In the Rule of Thirteen first, a base formula is generated which consists of only hydrogen and carbon atoms. This base formula is calculated by dividing the molecular mass by 13 (C + H: 12+ 1 =13). When a molecular mass, M+, is known, a base formula can be generated from the following.

What is base peak and molecular ion peak?

Base peak: The most intense (tallest) peak in a mass spectrum, due to the ion with the greatest relative abundance (relative intensity; height of peak along the spectrum's y-axis). Not to be confused with molecular ion: base peaks are not always molecular ions, and molecular ions are not always base peaks.

What is M+ in mass spectrometry?

The M+ peak is usually the highest intensity peak in the cluster of peaks at highest m/z.

Does a mass spectrometer separate isotopes?

Isotopes of an element have different charge to mass ratio. Mass spectrometers separate the isotopes of different element based on their mass divided by electric charge.

Is PF mandatory for salary below 15000?

EPF eligibility criteria If you are a salaried employee with a (basic + dearness allowance) less than Rs. 15,000 per month, it is mandatory for you to be opened an EPF account by your employer.

What is PF & ESI rules?

As per the act, all employees whose earnings are Rs. 15000 or less per month, it is required for them to contribute 0.75% of their pay towards the ESI and 3.25% by the company towards ESI. For successful , ESI Registration the employer will need to pay the advance contribution for 6 months.

Is ESI mandatory for salary above 21000?

So, the take-home salary of employees earning between Rs 15,000 to Rs 21,000 decreases as the employee contribution of 1.75% deducts from their salaries. Once an employee starts earning a wage of more than Rs 21,000, ESI contributions will be made only till the end of the contribution period. It is a 6-month period.

How do I find my ESI number?

Step 1:- To find the ESIC number of employees employers need to log in to their employer ESIC portal with their establishment id and password. Step 2:- On the home page of the employer ESIC portal there is an option called list of employees, now click on that.

Is gratuity and PF same?

Unlike employee provident fund which includes employee's contribution, the gratuity amount is entirely paid by the employer. Under the Payment of Gratuity Act, 1972 a certain percentage of the salary is calculated and deposited in a gratuity account payable later.

How basic salary is calculated?

Ideally, they use a reversed calculation method where a percentage of the salary and CTC is taken. The basic pay is usually 40% of gross income or 50% of an individual's CTC. Basic salary = Gross pay- total allowances (medical insurance, HRA, DA, conveyance, etc.)

Who can get ESIC benefits?

An individual who is employed in a non-seasonal factory that has more than 10 employees. This criteria applies under Section 2 (12) of the Act. With effect from 1 January 2017, the wage limit of an employee is set at Rs. 21,000 per month for him/her to come under the coverage of the ESI scheme.

What is the salary limit for ESI 2021?

All employees of a covered unit, whose monthly incomes (excluding overtime, bonus, leave encashment) does not exceed Rs. 21,000 per month, are eligible to avail benefits under the Scheme. Employees earning daily average wage up to Rs. 176 are exempted from ESIC contribution.

Can I withdraw ESI amount?

ESI is a premium paid for medical benefits. If no benefit is obtained from it, there is no option available to withdraw amount from ESI Account.

Can I continue ESI after leaving job?

In order to reactivate your ESI Pehchan card, you need to give your old ESIC number to your new employer. Your ESIC card will be reactivated when your new employer pays your ESIC contribution.

Is ESI deducted from basic salary?

As per the latest rules laid out by ESIC, the employees get 0.75% deducted from their respective gross salaries, whereas the employers make an ESI contribution of 3.25% of the employee's gross pay towards ESI.

What is the minimum number of employees for ESI?

The ESI Act is applicable to all non-seasonal factories employing 10 or more persons [Section -1(2) read with Section 2(12) and Section 2(19A)].

Can I claim ESI in private hospital?

Beneficiaries of the ESI health insurance scheme can get medical care at nearby private hospitals empanelled with the Employees' State Insurance Corporation in case of non-availability of ESIC hospitals within 10 kms of their residence, an official statement said.

Does ESI cover death?

Employees' State Insurance Scheme of India, is an integrated social security scheme tailored to provide social protection to workers in the organised sector and their dependants in contingencies, such as, sickness, maternity or death and disablement due to an employment injury or occupational disease.

What is ESIC pension?

Labour ministry notifies monthly pension relief of Rs 1,800 for dependents of ESIC beneficiaries. Agencies. In case of a legitimate or adopted son and a daughter, an amount equivalent to two-fifths of the full rate until he attains the age of twenty-five years shall be paid, among others.

Can I claim ESI for my wife?

wife can avail medical benefit from ESI hospitals/dispensaries. I.P. can also claim transport allowance if his residence is far away to ESI Hospital at the time of delivery. To get medical benefit it is necessary that her name is included in ESI card.

How do I claim my ESI hospital bill?

To submit a re-imbursement claim bill/bills an IP has to write an application to the Administrative Medical Officer, ESI Scheme, Assam through the concerned Insurance Medical Officer i/c, ESI Scheme Dispensary along with the claim bill format which is available at Dispensary. They have to mention the cause of diseases.

Is family covered under ESI?

The insured person and his family are entitled to the Medical Benefit from the very first day of his/her joining the insurable employment. A person who is just covered under the scheme for the first time is eligible for primary and secondary medical care for self and family for three months.

What is SAG formula?

The maximum dip (sag) is represented by the value of y at either of the supports A and B.At support A, x = l/2 and y = S. (ii) When supports are at unequal levels: In hilly areas, we generally come across conductors suspended between supports at unequal levels.

At what voltage does corona start?

On sharp points in the air, corona can start at potentials of 2–6 kV. In order to suppress corona formation, terminals on high voltage equipment are frequently designed with smooth large-diameter rounded shapes like balls or toruses.

What is Ferranti & corona effect?

Ferranti Effect • The Ferranti effect is a phenomenon that describes the increase in voltage at the receiving end of a long transmission line relative to the voltage at the sending end when the lines are either lightly loaded or open circuit. • Ferranti effect is due to the charging current of the line.

What is corona in transmission?

Corona Effect in Overhead Transmission LineThe phenomenon of violet glow, hissing sound and production of ozone gas in an overhead transmission line is known as corona. When a very high voltage is applied across two conductors of the overhead transmission line whose spacing is large as compared to their diameters.

What is corona in electrical?

DEFINITION: The ionization of air surrounding the high voltage transmission lines causing the conductors to glow, producing a hissing noise, is called Corona Discharge or Corona Effect.

What is sag in transmission line?

Sag literally means to be bending in shape. In. overhead transmission lines, the difference in level. between points of supports (towers or utility poles) and the lowest point on the conductor is called sag.